YcgT

From SubtiWiki
Revision as of 11:38, 1 April 2015 by Jstuelk (talk | contribs)
Jump to: navigation, search
  • Description: NADPH:ferredoxin oxidoreductase

Gene name ycgT
Synonyms
Essential no
Product NADPH:ferredoxin oxidoreductase
Function unknown
Gene expression levels in SubtiExpress: ycgT
MW, pI 36 kDa, 5.61
Gene length, protein length 1008 bp, 336 aa
Immediate neighbours ycgS, nasF
Sequences Protein DNA DNA_with_flanks
Genetic context
YcgT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YcgT expression.png















Categories containing this gene/protein

electron transport/ other

This gene is a member of the following regulons

Fur regulon

The gene

Basic information

  • Locus tag: BSU03270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH (according to Swiss-Prot)
  • Protein family: ferredoxin--NADP reductase type 2 family (according to Swiss-Prot)
  • Paralogous protein(s): YumC

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. PubMed

Expression and regulation

  • Operon: ycgT (according to DBTBS)
  • Regulation: induced under conditions of iron starvation (Fur) PubMed
  • Regulatory mechanism: Fur: transcription repression PubMed
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1152 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 572 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 617 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 256 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 84 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References