Difference between revisions of "YcgT"

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(Expression and regulation)
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* '''Description:''' similar to thioredoxin reductase <br/><br/>
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* '''Description:''' unknown <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || thioredoxin reductase (NADPH)
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|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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=== Additional information===
 
=== Additional information===
 +
The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin
 +
reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is availableavailable. {{PubMed|19935659}}
  
 
=Expression and regulation=
 
=Expression and regulation=

Revision as of 11:47, 26 November 2009

  • Description: unknown

Gene name ycgT
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 36 kDa, 5.61
Gene length, protein length 1008 bp, 336 aa
Immediate neighbours ycgS, nasF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcgT context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU03270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH (according to Swiss-Prot)
  • Protein family: ferredoxin--NADP reductase type 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is availableavailable. PubMed

Expression and regulation

  • Operon: ycgT (according to DBTBS)
  • Regulation: induced under conditions of iron starvation (Fur) PubMed
  • Regulatory mechanism: Fur: transcription repression PubMed
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)