YcbK

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  • Description: similar to efflux system

Gene name ycbK
Synonyms
Essential no
Product unknown
Function unknown
Metabolic function and regulation of this protein in SubtiPathways:
Phe, Tyr, Trp
MW, pI 33 kDa, 9.572
Gene length, protein length 936 bp, 312 aa
Immediate neighbours rtpA, ycbL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcbK context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

transporters/ other, membrane proteins

This gene is a member of the following regulons

T-box, TRAP regulon

The gene

Basic information

  • Locus tag: BSU02540

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: DUF6 domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • rtpA: induced by tryptophan limitation (T-box in the mRNA leader) PubMed
    • ycbK: induced by tryptophan limitation (MtrB in the mRNA leader) PubMed
  • Regulatory mechanism:
    • T-box: transcription termination/ antitermination PubMed
    • MtrB: control of translation initiation (access of the ribosome to the Shine-Dalgarno sequence) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Helen Yakhnin, Alexander V Yakhnin, Paul Babitzke
The trp RNA-binding attenuation protein (TRAP) of Bacillus subtilis regulates translation initiation of ycbK, a gene encoding a putative efflux protein, by blocking ribosome binding.
Mol Microbiol: 2006, 61(5);1252-66
[PubMed:16879415] [WorldCat.org] [DOI] (P p)

J P Sarsero, E Merino, C Yanofsky
A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis.
Proc Natl Acad Sci U S A: 2000, 97(6);2656-61
[PubMed:10706627] [WorldCat.org] [DOI] (P p)