Difference between revisions of "YcbK"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[rtpA]]-[[ycbK]]'' {{PubMed|10706627}}
  
* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]  
+
* '''[[Sigma factor]]:'''  
 +
** ''[[rtpA]]'': [[SigA]] {{PubMed|10706627}}
 +
** ''[[ycbK]]'': [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
  
* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
+
* '''Regulation:'''  
 +
** ''[[rtpA]]'': induced by tryptophan limitation ([[T-box]] in the mRNA leader) {{PubMed|10706627}}
 +
** ''[[ycbK]]'': induced by tryptophan limitation ([[MtrB]] in the mRNA leader) {{PubMed|10706627}}
 +
      induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[T-box]]: transcription termination/ antitermination {{PubMed|10706627}}
 +
** [[MtrB]]: transcription termination/ antitermination {{PubMed|10706627}}
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 10:03, 2 August 2009

  • Description: similar to efflux system

Gene name ycbK
Synonyms
Essential no
Product unknown
Function unknown
Metabolic function and regulation of this protein in SubtiPathways:
Phe, Tyr, Trp
MW, pI 33 kDa, 9.572
Gene length, protein length 936 bp, 312 aa
Immediate neighbours rtpA, ycbL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcbK context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU02540

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: DUF6 domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • rtpA: induced by tryptophan limitation (T-box in the mRNA leader) PubMed
    • ycbK: induced by tryptophan limitation (MtrB in the mRNA leader) PubMed
      induced by stress (SigB) PubMed
  • Regulatory mechanism:
    • T-box: transcription termination/ antitermination PubMed
    • MtrB: transcription termination/ antitermination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

J P Sarsero, E Merino, C Yanofsky
A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis.
Proc Natl Acad Sci U S A: 2000, 97(6);2656-61
[PubMed:10706627] [WorldCat.org] [DOI] (P p)