ycbH

168

galactarate dehydratase

locus: BSU_02510

Molecular weight: 54.63 kDa

pI: 5.7

Protein length: 510 aa

Gene length: 1533 bp

function: galactarate utilization

product: galactarate dehydratase

essential: no

ec: 4.2.1.42

synonyms: ycbH, garD

List of homologs in different organisms, belongs to COG2721 (Galperin et al., 2021)

Gene

Coordinates: 274,029 → 275,561

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The protein

Catalyzed reaction/ biological activity

• galactarate --> 5-dehydro-4-deoxy-D-glucarate + H₂O (according to UniProt)

Protein family

• uxaA family (with [protein|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA], according to UniProt)

Structure

• [PDB|6U7L] (from E. coli, 67.6% identity)
• [AF|P42240]

Biological materials

Mutant

• MGNA-C031 (ycbH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2029 NBRP B. subtilis, Japan]
• BKE02510 (Δ[gene|BA556E144975921DFDD598B0A7480C65176F0F14|ycbH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGAAAATCACTCCTCA,  downstream forward: _UP4_TGATTCATGTTTTTATAGCG
• BKK02510 (Δ[gene|BA556E144975921DFDD598B0A7480C65176F0F14|ycbH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGAAAATCACTCCTCA,  downstream forward: _UP4_TGATTCATGTTTTTATAGCG

References

• 12044674