YcbD

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  • Description: NADP(+)-dependent alpha-ketoglutaric semialdehyde dehydrogenase

Gene name ycbD
Synonyms
Essential no
Product NADP(+)-dependent alpha-ketoglutaric semialdehyde dehydrogenase
Function utilization of D-glucarate/galactarate
Gene expression levels in SubtiExpress: ycbD
MW, pI 52 kDa, 4.787
Gene length, protein length 1464 bp, 488 aa
Immediate neighbours ycbC, ycbE
Sequences Protein DNA DNA_with_flanks
Genetic context
YcbD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YcbD expression.png
























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YcbG regulon

The gene

Basic information

  • Locus tag: BSU02470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: an aldehyde + NAD+ + H2O = an acid + NADH (according to Swiss-Prot)
  • Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 36% identity, 69% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: induced by D-glucarate/galactarate (YcbG) PubMed
  • Regulatory mechanism: YcbG: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Seiya Watanabe, Masaki Yamada, Iwao Ohtsu, Keisuke Makino
alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution.
J Biol Chem: 2007, 282(9);6685-95
[PubMed:17202142] [WorldCat.org] [DOI] (P p)

Shigeo Hosoya, Kunio Yamane, Michio Takeuchi, Tsutomu Sato
Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.
FEMS Microbiol Lett: 2002, 210(2);193-9
[PubMed:12044674] [WorldCat.org] [DOI] (P p)