YcbD

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  • Description: NADP(+)-dependent alpha-ketoglutaric semialdehyde dehydrogenase

Gene name ycbD
Synonyms
Essential no
Product NADP(+)-dependent alpha-ketoglutaric semialdehyde dehydrogenase
Function utilization of D-glucarate/galactarate
MW, pI 52 kDa, 4.787
Gene length, protein length 1464 bp, 488 aa
Immediate neighbours ycbC, ycbE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcbD context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YcbG regulon

The gene

Basic information

  • Locus tag: BSU02470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: an aldehyde + NAD+ + H2O = an acid + NADH (according to Swiss-Prot)
  • Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 36% identity, 69% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: induced by D-glucarate/galactarate (YcbG) PubMed
  • Regulatory mechanism: YcbG: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Seiya Watanabe, Masaki Yamada, Iwao Ohtsu, Keisuke Makino
alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution.
J Biol Chem: 2007, 282(9);6685-95
[PubMed:17202142] [WorldCat.org] [DOI] (P p)

Shigeo Hosoya, Kunio Yamane, Michio Takeuchi, Tsutomu Sato
Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.
FEMS Microbiol Lett: 2002, 210(2);193-9
[PubMed:12044674] [WorldCat.org] [DOI] (P p)