ycbD

168

NADP⁺-dependent alpha-ketoglutaric semialdehyde dehydrogenase

locus: BSU_02470

Molecular weight: 52.25 kDa

pI: 4.79

Protein length: 488 aa

Gene length: 1467 bp

function: utilization of D-glucarate/galactarate

product: NADP⁺-dependent alpha-ketoglutaric semialdehyde dehydrogenase

essential: no

ec: 1.2.1.3

synonyms: ycbD

List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)

Gene

Coordinates: 268,846  270,312

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The protein

Catalyzed reaction/ biological activity

• 2,5-dioxopentanoate + H₂O + NADP⁺ --> 2-oxoglutarate + 2 H⁺ + NADPH (according to UniProt)

Protein family

• [wiki|aldehyde dehydrogenase family] (according to UniProt)

[wiki|Cofactors]

• NAD⁺

Structure

• [PDB|3RHH] (from from ''Bacillus halodurans'' C-125 complexed with NADP, 36% identity, 69% similarity)
• [AF|P42236]

Paralogous protein(s)

• [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|dhaS], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA], [protein|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA], [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|ywdH], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|gabD], [protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|aldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|aldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]

Biological materials

Mutant

• BKE02470 ([gene|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCCATTGCCCCTTTCG,  downstream forward: _UP4_TAATGGTGCTGGAAAGAGGC
• BKK02470 ([gene|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCCATTGCCCCTTTCG,  downstream forward: _UP4_TAATGGTGCTGGAAAGAGGC

References

• 12044674,17202142