ybfQ

168

unknown

locus: BSU_02330

Molecular weight: 37.11 kDa

pI: 4.91

Protein length: 322 aa

Gene length: 969 bp

function: unknown

product: unknown

essential: no

synonyms: ybfQ, trhO

List of homologs in different organisms, belongs to COG1054 (Galperin et al., 2021)

Gene

Coordinates: 252,514 → 253,482

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The protein

Protein family

• UPF0176 family (single member, according to UniProt)

[wiki|Domains]

• [wiki|Rhodanese domain] (aa 125-219) (according to UniProt)

Structure

• [PDB|4F67] (from Legionella pneumophila, corresponds to aa 10 ... 234, 41% identity)
• [AF|O31457]

Expression and Regulation

Operons

• genes: [gene|BA60CF44ECA4937FA5C1720B10E7FDABA9AB4664|ybfO]-[gene|1EE8BF589ECDFD5194C86E02A627CCF81899B904|ybfP]-[gene|E06787B6AAD11070751938822F75359CE6EF299C|ybfQ]

  description: [Pubmed|12207695]

  regulation

  • repressed during logarithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]) [Pubmed|18840696]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|18840696,20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|11866510,12207695], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]

Biological materials

Mutant

• MGNA-B936 (ybfQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1935 NBRP B. subtilis, Japan]
• BKE02330 (Δ[gene|E06787B6AAD11070751938822F75359CE6EF299C|ybfQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTAAACACCCTGTT,  downstream forward: _UP4_TAAAAAAAGAACACCTCGTA
• BKK02330 (Δ[gene|E06787B6AAD11070751938822F75359CE6EF299C|ybfQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTAAACACCCTGTT,  downstream forward: _UP4_TAAAAAAAGAACACCTCGTA

References

• 12207695