skfB

168

thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|spore killing factor]

locus: BSU_01920

Molecular weight: 45.59 kDa

pI: 4.93

Protein length: 410 aa

Gene length: 1233 bp

function: maturation of [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|spore killing factor]

product: AdoMet radical sactisynthase

essential: no

synonyms: skfB, ybcP, ybcQ

List of homologs in different organisms, belongs to COG0535 (Galperin et al., 2021)

Gene

Coordinates: 214,175 → 215,407

Advertisement

The protein

Catalyzed reaction/ biological activity

• generates a thioether bond in [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA], which links the cysteine residue Cys4 with the α-carbon of the methionine residue Met12 [Pubmed|23282011]

Protein family

• [wiki|Radical SAM superfamily] (according to UniProt)

[wiki|Cofactors]

• [4Fe-4S] and [2Fe-2S] clusters [Pubmed|30217813,23282011]

Structure

• [PDB|6EFN] [Pubmed|30217813]
• [AF|O31423]

Biological materials

Mutant

• MGNA-C513 (ybcQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2511 NBRP B. subtilis, Japan]
• BKE01920 (Δ[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATAAAATCCCCCTTAAG,  downstream forward: _UP4_GAAGTCAAGGCAGGGATTCA
• BKK01920 (Δ[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATAAAATCCCCCTTAAG,  downstream forward: _UP4_GAAGTCAAGGCAGGGATTCA

References

Reviews

• 20955377

Research papers

• 23282011,14651647,15687200,17720793,16816204,16452424,30217813