yaaO

yaaO
168

similar to arginine decarboxylase, affects the level of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] modification

locus
BSU_00270
Molecular weight
53.00 kDa
pI
5.71
Protein length
Gene length
function
control of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] modification
product
putative arginine decarboxylase
essential
no
ec
4.1.1.19
synonyms
yaaO

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1982 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
37,720 → 39,162
Phenotypes of a mutant
inactivation suppresses the swarming defect of a [gene|248F0805272FED9B38ECBB31E2872BC9EC163CE0|ymfI] mutant [pubmed|29615499]
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The protein
Catalyzed reaction/ biological activity
the protein was reported to be involved in norspermidine production and biofilm disassembly [Pubmed|22541437]; however, this is not the case [Pubmed|24529384] and the original paper has been [http://www.cell.com/cell/fulltext/S0092-8674(15)00495-X retracted]
Protein family
Orn/Lys/Arg decarboxylase class-I family (together with [protein|03E5E41FD7ADA4E9CB70E312FA5F163331C92695|speA]) (according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|2X3L] (from ''Staphylococcus aureus'', 36% identity) [Pubmed|20419351]
[AF|P37536]
Paralogous protein(s)
[protein|03E5E41FD7ADA4E9CB70E312FA5F163331C92695|speA]
Expression and Regulation
Operons
genes
[gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]-[gene|5231A0EE9C5A7556817B686D5B2B7B72B675BCAC|yaaN]-[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]-[gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
description
[Pubmed|22383849]
Open in new tab

[gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]→[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]

2024-03-28 12:47:40

Jstuelk

136

adcd0181c3bf7f5a66d1644d176d0c60f2121a3a

87CEB0D67D1455F48A42F665A0B9A91B779AEC5D

genes
[gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]-[gene|5231A0EE9C5A7556817B686D5B2B7B72B675BCAC|yaaN]-[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]
description
[Pubmed|22383849]
regulation
expressed in stationary phase
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|9987136], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|22383849], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
Open in new tab

[gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]→[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]

2024-03-10 08:59:17

ghost

88

7abb07cd575ba41a995431f6ebc94468beb3ee78

DCAF5B7EFE6C076A4C00A688D242AC2F436F8CAC

Biological materials
Mutant
GP3961 (Δ[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]::kan), available in [wiki|Jörg Stülke]'s lab
GP3962 (Δ[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]::phleo), available in [wiki|Jörg Stülke]'s lab
MGNA-B896 (yaaO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1895 NBRP B. subtilis, Japan]
BKE00270 (Δ[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAAACAATCCTTTGAA,  downstream forward: _UP4_GTTTATATAGAAGAGGAGAA
BKK00270 (Δ[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAAACAATCCTTTGAA,  downstream forward: _UP4_GTTTATATAGAAGAGGAGAA
Expression vectors
pGP3751: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
pGP3753: expression of Strep-''yaaO'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3754: expression of ''yaaO''-Strep by [wiki|pGP382] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3757 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
References
24529384,20876533,22541437,22383849,9987136,20419351,29615499

EEF3E572ED2F23581EB9B48D16B3112B886F7975

Page visits: 5477

Time of last update: 2024-03-28 15:25:43

Author of last update: Robert.warneke