XylA

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  • Description: xylose isomerase

Gene name xylA
Synonyms
Essential no
Product xylose isomerase
Function utilization of xylan and xylose
Gene expression levels in SubtiExpress: xylA
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 50 kDa, 5.635
Gene length, protein length 1335 bp, 445 aa
Immediate neighbours xylR, xylB
Sequences Protein DNA Advanced_DNA
Genetic context
XylA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
XylA expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, XylR regulon

The gene

Basic information

  • Locus tag: BSU17600

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-xylose = D-xylulose (according to Swiss-Prot)
  • Protein family: xylose isomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1A0D (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant: GP1151 (del aphA3) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage

Your additional remarks

References

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

M K Dahl, J Degenkolb, W Hillen
Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs.
J Mol Biol: 1994, 243(3);413-24
[PubMed:7966270] [WorldCat.org] [DOI] (P p)

A Kraus, C Hueck, D Gärtner, W Hillen
Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.
J Bacteriol: 1994, 176(6);1738-45
[PubMed:8132469] [WorldCat.org] [DOI] (P p)

D Gärtner, J Degenkolb, J A Ripperger, R Allmansberger, W Hillen
Regulation of the Bacillus subtilis W23 xylose utilization operon: interaction of the Xyl repressor with the xyl operator and the inducer xylose.
Mol Gen Genet: 1992, 232(3);415-22
[PubMed:1588910] [WorldCat.org] [DOI] (P p)

S Jacob, R Allmansberger, D Gärtner, W Hillen
Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame.
Mol Gen Genet: 1991, 229(2);189-96
[PubMed:1921970] [WorldCat.org] [DOI] (P p)

D Gärtner, M Geissendörfer, W Hillen
Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.
J Bacteriol: 1988, 170(7);3102-9
[PubMed:2454911] [WorldCat.org] [DOI] (P p)