XylA

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  • Description: xylose isomerase

Gene name xylA
Synonyms
Essential no
Product xylose isomerase
Function utilization of xylan and xylose
Gene expression levels in SubtiExpress: xylA
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 50 kDa, 5.635
Gene length, protein length 1335 bp, 445 aa
Immediate neighbours xylR, xylB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
XylA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
XylA expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, XylR regulon

The gene

Basic information

  • Locus tag: BSU17600

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-xylose = D-xylulose (according to Swiss-Prot)
  • Protein family: xylose isomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1A0D (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant: GP1151 (del aphA3) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage

Your additional remarks

References

M K Dahl, J Degenkolb, W Hillen
Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs.
J Mol Biol: 1994, 243(3);413-24
[PubMed:7966270] [WorldCat.org] [DOI] (P p)

A Kraus, C Hueck, D Gärtner, W Hillen
Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.
J Bacteriol: 1994, 176(6);1738-45
[PubMed:8132469] [WorldCat.org] [DOI] (P p)

D Gärtner, J Degenkolb, J A Ripperger, R Allmansberger, W Hillen
Regulation of the Bacillus subtilis W23 xylose utilization operon: interaction of the Xyl repressor with the xyl operator and the inducer xylose.
Mol Gen Genet: 1992, 232(3);415-22
[PubMed:1588910] [WorldCat.org] [DOI] (P p)

S Jacob, R Allmansberger, D Gärtner, W Hillen
Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame.
Mol Gen Genet: 1991, 229(2);189-96
[PubMed:1921970] [WorldCat.org] [DOI] (P p)

D Gärtner, M Geissendörfer, W Hillen
Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.
J Bacteriol: 1988, 170(7);3102-9
[PubMed:2454911] [WorldCat.org] [DOI] (P p)