Difference between revisions of "XylA"
| Line 92: | Line 92: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[xylA]]-[[xylB]]'' | + | * '''Operon:''' ''[[xylA]]-[[xylB]]'' {{PubMed|1921970}} |
| − | * '''[[Sigma factor]]:''' [[SigA]] | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2454911}} |
| − | * '''Regulation:''' | + | * '''Regulation:''' |
| + | ** carbon catabolite repression ([[CcpA]]) {{PubMed|8132469}} | ||
| + | ** induction by xylose ([[XylR]]) {{PubMed|2454911}} | ||
| − | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression | + | * '''Regulatory mechanism:''' |
| + | ** [[CcpA]]: transcription repression {{PubMed|8132469}} | ||
| + | ** [[XylR]]: transcription repression {{PubMed|2454911}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 124: | Line 128: | ||
=References= | =References= | ||
| − | <pubmed>7966270,, | + | <pubmed>7966270,,1921970 8132469 2454911 1588910, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:48, 18 October 2009
- Description: xylose isomerase
| Gene name | xylA |
| Synonyms | |
| Essential | no |
| Product | xylose isomerase |
| Function | utilization of xylan and xylose |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 50 kDa, 5.635 |
| Gene length, protein length | 1335 bp, 445 aa |
| Immediate neighbours | xylR, xylB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU17600
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-xylose = D-xylulose (according to Swiss-Prot)
- Protein family: xylose isomerase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 1A0D (Geobacillus stearothermophilus)
- UniProt: P04788
- KEGG entry: [3]
- E.C. number: 5.3.1.5
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Wolfgang Hillen, Erlangen University, Germany Homepage
Your additional remarks
References
M K Dahl, J Degenkolb, W Hillen
Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs.
J Mol Biol: 1994, 243(3);413-24
[PubMed:7966270]
[WorldCat.org]
[DOI]
(P p)
A Kraus, C Hueck, D Gärtner, W Hillen
Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.
J Bacteriol: 1994, 176(6);1738-45
[PubMed:8132469]
[WorldCat.org]
[DOI]
(P p)
D Gärtner, J Degenkolb, J A Ripperger, R Allmansberger, W Hillen
Regulation of the Bacillus subtilis W23 xylose utilization operon: interaction of the Xyl repressor with the xyl operator and the inducer xylose.
Mol Gen Genet: 1992, 232(3);415-22
[PubMed:1588910]
[WorldCat.org]
[DOI]
(P p)
S Jacob, R Allmansberger, D Gärtner, W Hillen
Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame.
Mol Gen Genet: 1991, 229(2);189-96
[PubMed:1921970]
[WorldCat.org]
[DOI]
(P p)
D Gärtner, M Geissendörfer, W Hillen
Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.
J Bacteriol: 1988, 170(7);3102-9
[PubMed:2454911]
[WorldCat.org]
[DOI]
(P p)
