Difference between revisions of "Xpt"

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(Database entries)
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* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** repressed in the presence of purine nucleotides ([[PurR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11591660 PubMed]
 
** repressed in the presence of adenine or adenosine ([[PurR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11591660 PubMed]
 
** repressed in the presence of adenine or adenosine ([[PurR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11591660 PubMed]
 
** induced in the absence of guanine ([[G-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed]
 
** induced in the absence of guanine ([[G-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[PurR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/11591660 PubMed]
 
** [[G-box]]: transcription termination/ antitermination  ([[riboswitch]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098051 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]  
 
** [[G-box]]: transcription termination/ antitermination  ([[riboswitch]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098051 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]  
 
** [[PurR]]: transcription repression (molecular inducer: PRPP) [http://www.ncbi.nlm.nih.gov/sites/entrez/11591660 PubMed]
 
** [[PurR]]: transcription repression (molecular inducer: PRPP) [http://www.ncbi.nlm.nih.gov/sites/entrez/11591660 PubMed]

Revision as of 17:10, 11 June 2009

  • Description: xanthine phosphoribosyltransferase

Gene name xpt
Synonyms
Essential no
Product xanthine phosphoribosyltransferase
Function purine salvage and interconversion
MW, pI 20 kDa, 5.835
Gene length, protein length 582 bp, 194 aa
Immediate neighbours pbuX, ypwA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Xpt context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU22070

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 1Y0B, 2FXV (complex with GMP), 1Y27 (the riboswitch in complex with guanine)
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • repressed in the presence of adenine or adenosine (PurR) PubMed
    • induced in the absence of guanine (G-box) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed