xpt

168

xanthine phosphoribosyltransferase

locus: BSU_22070

Molecular weight: 20.89 kDa

pI: 5.84

Protein length: 194 aa

Gene length: 585 bp

function: purine salvage and interconversion

product: xanthine phosphoribosyltransferase

essential: no

ec: 2.4.2.22

synonyms: xpt

List of homologs in different organisms, belongs to COG0503 (Galperin et al., 2021)

Gene

Coordinates: 2,319,440 → 2,320,024

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The protein

Catalyzed reaction/ biological activity

• 5-phospho-α-D-ribose 1-diphosphate + xanthine --> diphosphate + XMP (according to UniProt)

Protein family

• [wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)

Structure

• [PDB|1Y0B],  [PDB|2FXV] (complex with GMP), [PDB|1Y27] (the riboswitch in complex with guanine)
• [AF|P42085]

Effectors of protein activity

• strongly inhibited by pGpp and ppGpp [pubmed|32446804]
• weakly inhibited by pppGpp [pubmed|32446804]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Biological materials

Mutant

• MGNA-A870 (xpt::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/870 NBRP B. subtilis, Japan]
• BKE22070 ([gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTGTCTACCTCCGTTA,  downstream forward: _UP4_TCCTTCGTACAGGAGGTTCA
• BKK22070 ([gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTGTCTACCTCCGTTA,  downstream forward: _UP4_TCCTTCGTACAGGAGGTTCA

lacZ fusion

• pGP430 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab

References

• 16716072,9098051,12923093,15629952,11591660,15610857,20200045,11591660,20204714,26704707,27941758,28541183,28798404,32446804,35622174