xpt

xpt
168

xanthine phosphoribosyltransferase

locus
BSU_22070
Molecular weight
20.89 kDa
pI
5.84
Protein length
Gene length
function
purine salvage and interconversion
product
xanthine phosphoribosyltransferase
essential
no
ec
2.4.2.22
synonyms
xpt

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0503 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,319,440 → 2,320,024
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The protein
Catalyzed reaction/ biological activity
5-phospho-α-D-ribose 1-diphosphate + xanthine --> diphosphate + XMP (according to UniProt)
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
Structure
[PDB|1Y0B],  [PDB|2FXV] (complex with GMP), [PDB|1Y27] (the riboswitch in complex with guanine)
[AF|P42085]
Effectors of protein activity
strongly inhibited by pGpp and ppGpp [pubmed|32446804]
weakly inhibited by pppGpp [pubmed|32446804]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]-[gene|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|pbuX]
description
[Pubmed|9098051]
regulation
induced in the absence of guanine ([wiki|G-box]) [Pubmed|12787499]
regulatory mechanism
G-box: RNA switch, [wiki|riboswitch] [Pubmed|9098051,12787499], in [regulon|other_regulator:G-box|G-box]
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|11591660], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9098051], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]→[gene|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|pbuX]

2024-03-14 12:06:55

Jstuelk

66

e2aea9be4482d2f1a4ec3d17e057d345c5db5dbf

D9AD2007FF8AC8985A79EBB6FAAF4F457CB0852B

Biological materials
Mutant
MGNA-A870 (xpt::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/870 NBRP B. subtilis, Japan]
BKE22070 ([gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTGTCTACCTCCGTTA,  downstream forward: _UP4_TCCTTCGTACAGGAGGTTCA
BKK22070 ([gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTGTCTACCTCCGTTA,  downstream forward: _UP4_TCCTTCGTACAGGAGGTTCA
lacZ fusion
pGP430 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
16716072,9098051,12923093,15629952,11591660,15610857,20200045,11591660,20204714,26704707,27941758,28541183,28798404,32446804,35622174

02C84C9A8C0008CC253CBF13584A4B5B2D2B510C

Page visits: 3880

Time of last update: 2024-03-28 13:55:01

Author of last update: Jstuelk