WapA

• Description: cell wall-associated protein precursor, contact-dependent growth inhibition protein
  
  

Gene name	wapA
Synonyms
Essential	no
Product	cell wall-associated protein precursor
Function	intercellular competition
Gene expression levels in SubtiExpress: wapA
Interactions involving this protein in SubtInteract: WapA
MW, pI	258 kDa, 6.131
Gene length, protein length	7002 bp, 2334 aa
Immediate neighbours	yxxG, yxxF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, toxins, antitoxins and immunity against toxins

This gene is a member of the following regulons

DegU regulon, PhoP regulon, WalR regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU39230

Database entries

• BsubCyc: BSU39230

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • the C-terminal toxic domain has RNase activity (cleaves tRNAs) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • cell wall binding domain as retention signal
  • C-terminal toxic domain with RNase activity (cleaves tRNAs) PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:
  • YxxG inhibits the toxic activity of WapA PubMed

• Interactions:
  • WapA-YxxG PubMed

• Localization:
  • extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome PubMed

Database entries

• BsubCyc: BSU39230

• Structure:

• UniProt: Q07833

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: wapA-yxxG PubMed

• Expression browser: wapA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed at high salt concentration (DegU-P) PubMed
  • repressed when phosphate is available (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • DegU-P: transcription repression PubMed
  • activated by YvrHb PubMed
  • WalR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 66 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 6 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 51 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: