Difference between revisions of "WapA"

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* '''Description:''' cell wall-associated protein precursor <br/><br/>
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* '''Description:''' cell wall-associated protein precursor, contact-dependent growth inhibition protein <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || cell wall-associated protein precursor
 
|style="background:#ABCDEF;" align="center"| '''Product''' || cell wall-associated protein precursor
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || intercellular competition
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39230 wapA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39230 wapA]
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/WapA WapA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 258 kDa, 6.131   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 258 kDa, 6.131   
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[cell wall/ other]]}}
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{{SubtiWiki category|[[cell wall/ other]]}},
 +
{{SubtiWiki category|[[toxins, antitoxins and immunity against toxins]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
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* '''Domains:''' cell wall binding domain as retention signal
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* '''Domains:'''  
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** cell wall binding domain as retention signal
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** C-terminal toxic domain with RNase activity (cleaves tRNAs) {{PubMed|23572593}}
  
 
* '''Modification:'''
 
* '''Modification:'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 +
** [[YxxG]] inhibits the toxic activity of [[WapA]] {{PubMed|23572593}}
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** [[WapA]]-[[YxxG]] {{PubMed|23572593}}
  
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* '''[[Localization]]:''' extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
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* '''[[Localization]]:'''  
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** extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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=References=
 
=References=
  
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<pubmed>11987133,16672620,18957862 16306698, 9537385 21821766</pubmed>
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<pubmed>11987133,16672620,18957862 16306698, 9537385 21821766 23572593 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:49, 19 April 2013

  • Description: cell wall-associated protein precursor, contact-dependent growth inhibition protein

Gene name wapA
Synonyms
Essential no
Product cell wall-associated protein precursor
Function intercellular competition
Gene expression levels in SubtiExpress: wapA
Interactions involving this protein in SubtInteract: WapA
MW, pI 258 kDa, 6.131
Gene length, protein length 7002 bp, 2334 aa
Immediate neighbours yxxG, yxxF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
WapA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
WapA expression.png















Categories containing this gene/protein

cell wall/ other, toxins, antitoxins and immunity against toxins

This gene is a member of the following regulons

DegU regulon, YvrHb regulon

The gene

Basic information

  • Locus tag: BSU39230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relexed selection for sporulation PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • cell wall binding domain as retention signal
    • C-terminal toxic domain with RNase activity (cleaves tRNAs) PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed at high salt concentration (DegU-P) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sanna Koskiniemi, James G Lamoureux, Kiel C Nikolakakis, Claire t'Kint de Roodenbeke, Michael D Kaplan, David A Low, Christopher S Hayes
Rhs proteins from diverse bacteria mediate intercellular competition.
Proc Natl Acad Sci U S A: 2013, 110(17);7032-7
[PubMed:23572593] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

Masakuni Serizawa, Keisuke Kodama, Hiroki Yamamoto, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses.
Biosci Biotechnol Biochem: 2005, 69(11);2155-69
[PubMed:16306698] [WorldCat.org] [DOI] (P p)

Haike Antelmann, Hiroki Yamamoto, Junichi Sekiguchi, Michael Hecker
Stabilization of cell wall proteins in Bacillus subtilis: a proteomic approach.
Proteomics: 2002, 2(5);591-602
[PubMed:11987133] [WorldCat.org] [DOI] (P p)

V Dartois, M Débarbouillé, F Kunst, G Rapoport
Characterization of a novel member of the DegS-DegU regulon affected by salt stress in Bacillus subtilis.
J Bacteriol: 1998, 180(7);1855-61
[PubMed:9537385] [WorldCat.org] [DOI] (P p)

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