walJ

walJ
168

5'-3' double-stranded DNA exonuclease, coordination of cell division with DNA replication

locus
BSU_40370
Molecular weight
29.77 kDa
pI
5.5
Protein length
Gene length
function
coordination of cell division with DNA replication
product
5'-3' double-stranded DNA exonuclease
essential
no
ec
3.-.-.-
synonyms
walJ, yycJ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1235 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,148,851 → 4,149,645
Phenotypes of a mutant
the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited [Pubmed|21169496]
increased sensitivity to a narrow spectrum of cephalosporin antibiotics [Pubmed|21169496]
disruption of the corresponding gene in B. anthracis results in a mutator phenotype [Pubmed|21205011]
improved survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]
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The protein
Catalyzed reaction/ biological activity
5'-3' exonuclease activity at the 5' termini and at nicks of double-stranded DNA [Pubmed|23491602]
the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of [wiki|cell division] with DNA replication [Pubmed|21169496]
Protein family
predicted member of the metallo-β-lactamase superfamily
[wiki|Cofactors]
Mn(II) [Pubmed|23491602]
Structure
[AF|C0SP91]
Expression and Regulation
Operons
genes
[gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
description
[Pubmed|9829949]
regulation
''htrC'' transcript expressed during sporulation [Pubmed|9829949]
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[gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]→[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]

2024-03-24 13:36:40

ghost

104

4e891bb3dceb8eda2e5ad4f9ed646a611afbe349

32BB8D419E54714C8925B07FBBCF691251CA4145

Biological materials
Mutant
GP3558 (Δ[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]::aphA3), available in [wiki|Jörg Stülke]'s lab
MGNA-B824 (yycJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1823 NBRP B. subtilis, Japan]
1A1044 ( ''walJ''::''spec''), [Pubmed|21169496], available at [BGhttp://bgsc.org/getdetail.php?bgscid=1A1044]
BKE40370 (Δ[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGTCTCACTCCATT,  downstream forward: _UP4_TAATTTTTCTATGTAAATCA
BKK40370 (Δ[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGTCTCACTCCATT,  downstream forward: _UP4_TAATTTTTCTATGTAAATCA
References
Reviews
26343983
Original Publications
9829949,21169496,21205011,23491602,36574412

D5321F707E183E72B225F1CED2F2FDCB1183B88A

Page visits: 3714

Time of last update: 2024-03-27 21:15:22

Author of last update: Jstuelk