vmlR

168

F-type ATP-binding cassette protein, allosterically dissociates antibiotics (virginiamycin M, lincomycin) from their ribosomal binding sites

locus: BSU_05610

Molecular weight: 62.57 kDa

pI: 6.73

Protein length: 547 aa

Gene length: 1644 bp

function: dissociation of antibiotics (virginiamycin M, lincomycin) from the ribosome

product: ABCF ATPase

essential: no

synonyms: vmlR, expZ

List of homologs in different organisms, belongs to COG0488 (Galperin et al., 2021)

Gene

Coordinates: 604,736  606,379

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)
• [wiki|ABCF ATPase subfamily] [pubmed|30597160]

[wiki|Domains]

• 2 [wiki|ABC transporter domain]s (aa 5-200, aa 292-504) (according to UniProt)

Structure

• [PDB|6HA8] [pubmed|30126986]
• [AF|P39115]

[wiki|Localization]

• cytoplasm [pubmed|30597160]

Biological materials

Mutant

• MGNA-C159 (expZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2157 NBRP B. subtilis, Japan]
• BKE05610 ([gene|032BF9280D006DD6D7C7D07A983C95E1433AC88C|vmlR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCATATCCCTCGCTTTA,  downstream forward: _UP4_TGAGGGAGAAAGTTCAAGCG
• BKK05610 ([gene|032BF9280D006DD6D7C7D07A983C95E1433AC88C|vmlR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCATATCCCTCGCTTTA,  downstream forward: _UP4_TGAGGGAGAAAGTTCAAGCG

References

Reviews

• 30746819

Original Publications

• 10092453,16109936,20525796,24687494,27120414,24389466,30126986,30597160,35699226