ValS

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  • Description: valyl-tRNA synthetase

Gene name valS
Synonyms
Essential yes PubMed
Product valyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: valS
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging, Folate
MW, pI 101 kDa, 4.868
Gene length, protein length 2640 bp, 880 aa
Immediate neighbours folC, yszA
Sequences Protein DNA Advanced_DNA
Genetic context
ValS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ValS expression.png















Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box, Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU28090

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val) (according to Swiss-Prot)
  • Protein family: ValS type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1IYW (from Thermus thermophilus, 41% identity, 60% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Original Publications

D Luo, J Leautey, M Grunberg-Manago, H Putzer
Structure and regulation of expression of the Bacillus subtilis valyl-tRNA synthetase gene.
J Bacteriol: 1997, 179(8);2472-8
[PubMed:9098041] [WorldCat.org] [DOI] (P p)