Difference between revisions of "UreB"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[utilization of nitrogen sources other than amino acids]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[CodY regulon]]}},
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{{SubtiWiki regulon|[[GlnR regulon]]}},
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{{SubtiWiki regulon|[[PucR regulon]]}},
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{{SubtiWiki regulon|[[SigH regulon]]}},
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{{SubtiWiki regulon|[[TnrA regulon]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[utilization of nitrogen sources other than amino acids]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 00:13, 9 December 2010

  • Description: urease (beta subunit)

Gene name ureB
Synonyms
Essential no
Product urease (beta subunit)
Function utilization of urea as alternative nitrogen source
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Ammonium/ glutamate
MW, pI 13 kDa, 9.751
Gene length, protein length 372 bp, 124 aa
Immediate neighbours ureC, ureA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
UreB context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

CodY regulon, GlnR regulon, PucR regulon, SigH regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU36650

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Urea + H2O = CO2 + 2 NH3 (according to Swiss-Prot)
  • Protein family: urease beta subunit family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by nitrogen limitation (GlnR, TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Jaclyn L Brandenburg, Lewis V Wray, Lars Beier, Hanne Jarmer, Hans H Saxild, Susan H Fisher
Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
J Bacteriol: 2002, 184(21);6060-4
[PubMed:12374841] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, S H Fisher
Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H.
J Bacteriol: 1997, 179(17);5494-501
[PubMed:9287005] [WorldCat.org] [DOI] (P p)