ureA

168

urease (gamma subunit)

locus: BSU_36660

Molecular weight: 11.32 kDa

pI: 4.98

Protein length: 105 aa

Gene length: 318 bp

function: utilization of urea as alternative nitrogen source

product: urease (gamma subunit)

essential: no

ec: 3.5.1.5

synonyms: ureA

List of homologs in different organisms, belongs to COG0831 (Galperin et al., 2021)

Gene

Coordinates: 3,768,791  3,769,108

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The protein

Catalyzed reaction/ biological activity

• 2 H⁺ + H₂O + urea --> CO₂ + 2 NH₄⁺ (according to UniProt)

Protein family

• urease gamma subunit family (single member, according to UniProt)

Structure

• [PDB|2FVH] (from ''Mycobacterium tuberculosis'', 57% identity, 62% similarity)
• [AF|P75030]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE36660 ([gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTAGTCCTCCTTCTT,  downstream forward: _UP4_CCAATTTCTGCGGAGGTGAA
• BKK36660 ([gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36660 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTAGTCCTCCTTCTT,  downstream forward: _UP4_CCAATTTCTGCGGAGGTGAA

References

Reviews

• 23539618,19363030

Original Publications

• 12374841,18083814,12618455,9287005,9287005,12374841,9287005,1670935,16199586,25755103