Difference between revisions of "UreA"

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(Expression and regulation)
Line 94: Line 94:
 
* '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
 
* '''[[Sigma factor]]:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
  
* '''Regulation:''' induced by nitrogen limitation ([[GlnR]], [[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed], repressed by [[CodY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
+
* '''Regulation:'''  
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
+
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]], [[GlnR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
 
** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12374841 PubMed]
 
** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12374841 PubMed]
 
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
 
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
  
* '''Regulatory mechanism:''' [[GlnR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed], [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed], [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed1]  [http://www.ncbi.nlm.nih.gov/sites/entrez/18083814 PubMed2]
+
* '''Regulatory mechanism:'''  
 +
** [[GlnR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
 
** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
 
** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
** [PucR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12374841 PubMed]
+
** [[PucR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12374841 PubMed]
 
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
 
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287005 PubMed]
  

Revision as of 10:17, 15 December 2009

  • Description: urease (gamma subunit)

Gene name ureA
Synonyms
Essential no
Product urease (gamma subunit)
Function utilization of urea as alternative nitrogen source
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Ammonium/ glutamate
MW, pI 11 kDa, 4.98
Gene length, protein length 315 bp, 105 aa
Immediate neighbours ureB, csbD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
UreA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU36660

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Urea + H2O = CO2 + 2 NH3 (according to Swiss-Prot)
  • Protein family: urease gamma subunit family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA, GlnR) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris R Belitsky, Abraham L Sonenshein
Genetic and biochemical analysis of CodY-binding sites in Bacillus subtilis.
J Bacteriol: 2008, 190(4);1224-36
[PubMed:18083814] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Jaclyn L Brandenburg, Lewis V Wray, Lars Beier, Hanne Jarmer, Hans H Saxild, Susan H Fisher
Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
J Bacteriol: 2002, 184(21);6060-4
[PubMed:12374841] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, S H Fisher
Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H.
J Bacteriol: 1997, 179(17);5494-501
[PubMed:9287005] [WorldCat.org] [DOI] (P p)

M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J Bacteriol: 1991, 173(1);23-7
[PubMed:1670935] [WorldCat.org] [DOI] (P p)