TyrZ

• Description: alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins
  
  

Gene name	tyrZ
Synonyms	ipa-9r, tyrS1, tyrT
Essential	no
Product	tyrosyl-tRNA synthetase (minor)
Function	translation
Gene expression levels in SubtiExpress: tyrZ
Metabolic function and regulation of this protein in SubtiPathways: tyrZ
MW, pI	46 kDa, 6.661
Gene length, protein length	1239 bp, 413 aa
Immediate neighbours	dtrR, ywaD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

DtrR regulon, T-box

The gene

Basic information

• Locus tag: BSU38460

Phenotypes of a mutant

• sensitive against D-Tyr PubMed
• a tyrS tyrZ double mutant is not viable PubMed

Database entries

• BsubCyc: BSU38460

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
  • TyrZ is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than TyrS PubMed

• Protein family: TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Thr-311 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU38460

• Structure:

• UniProt: P25151

• KEGG entry: [3]

• E.C. number: 6.1.1.1

Expression and regulation

• Operon: dtrR-tyrZ PubMed

• Expression browser: tyrZ PubMed

• Sigma factor: tyrZ PubMed

• Regulation:
  • induced by tyrosine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed
  • DtrR: transcriptional repression PubMed

• Additional information:

Biological materials

• Mutant: QB53532 (aphA3), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Rebecca N Williams-Wagner, Frank J Grundy, Medha Raina, Michael Ibba, Tina M Henkin
The Bacillus subtilis tyrZ gene encodes a highly selective tyrosyl-tRNA synthetase and is regulated by a MarR regulator and T box riboswitch.
J Bacteriol: 2015, 197(9);1624-31
[PubMed:25733610] [WorldCat.org] [DOI] (I p)

Boumediene Soufi, Chanchal Kumar, Florian Gnad, Matthias Mann, Ivan Mijakovic, Boris Macek
Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis.
J Proteome Res: 2010, 9(7);3638-46
[PubMed:20509597] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

T M Henkin, B L Glass, F J Grundy
Analysis of the Bacillus subtilis tyrS gene: conservation of a regulatory sequence in multiple tRNA synthetase genes.
J Bacteriol: 1992, 174(4);1299-306
[PubMed:1735721] [WorldCat.org] [DOI] (P p)