Difference between revisions of "TufA"

From SubtiWiki
Jump to: navigation, search
Line 30: Line 30:
  
 
<br/><br/>
 
<br/><br/>
 +
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[translation]]}},
 +
{{SubtiWiki category|[[essential genes]]}},
 +
{{SubtiWiki category|[[membrane proteins]]}},
 +
{{SubtiWiki category|[[phosphoproteins]]}}
 +
 +
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[stringent response]]}}
  
 
=The gene=
 
=The gene=
Line 51: Line 60:
  
  
= Categories containing this gene/protein =
+
 
{{SubtiWiki category|[[translation]]}},
 
{{SubtiWiki category|[[essential genes]]}},
 
{{SubtiWiki category|[[membrane proteins]]}},
 
{{SubtiWiki category|[[phosphoproteins]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 15:25, 8 December 2010

  • Description: elongation factor Tu

Gene name tufA
Synonyms
Essential yes PubMed
Product elongation factor Tu
Function translation
MW, pI 43 kDa, 4.723
Gene length, protein length 1188 bp, 396 aa
Immediate neighbours fusA, ybaC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
TufA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

translation, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01130

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: EF-Tu/EF-1A subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB PubMed, membrane PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP839, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP847, available in Stülke lab


  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References