Difference between revisions of "TufA"

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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
* '''Modification:''' phosphorylated on Thr-384 by [[PrkC]], dephosphorylated by [[PrpC]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
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* '''Modification:'''  
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** phosphorylated on Thr-384 by [[PrkC]], dephosphorylated by [[PrpC]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]
 
** Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species [http://www.ncbi.nlm.nih.gov/pubmed/22938038 PubMed]
 
** Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species [http://www.ncbi.nlm.nih.gov/pubmed/22938038 PubMed]
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** phosphorylated on several Arg residues {{PubMed|24263382}}
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tufA_132882_134072_1 tufA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tufA_132882_134072_1 tufA] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
  
<pubmed>22938038,19246764,20133608 , 19246764,  17726680, 18763711 </pubmed>
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<pubmed>22938038,19246764,20133608 , 19246764,  17726680, 18763711 24263382 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:52, 17 December 2013

  • Description: elongation factor Tu

Gene name tufA
Synonyms
Essential yes PubMed
Product elongation factor Tu
Function translation
Gene expression levels in SubtiExpress: tufA
Interactions involving this protein in SubtInteract: TufA
MW, pI 43 kDa, 4.723
Gene length, protein length 1188 bp, 396 aa
Immediate neighbours fusA, ybaC
Sequences Protein DNA DNA_with_flanks
Genetic context
TufA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TufA expression.png















Categories containing this gene/protein

translation, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01130

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: EF-Tu/EF-1A subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Thr-384 by PrkC, dephosphorylated by PrpC PubMed, PubMed
    • Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species PubMed
    • phosphorylated on several Arg residues PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP839, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP847, available in Stülke lab


  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Andreas Schmidt, Débora Broch Trentini, Silvia Spiess, Jakob Fuhrmann, Gustav Ammerer, Karl Mechtler, Tim Clausen
Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response.
Mol Cell Proteomics: 2014, 13(2);537-50
[PubMed:24263382] [WorldCat.org] [DOI] (I p)

Bui Khanh Chi, Alexandra A Roberts, Tran Thi Thanh Huyen, Katrin Bäsell, Dörte Becher, Dirk Albrecht, Chris J Hamilton, Haike Antelmann
S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria.
Antioxid Redox Signal: 2013, 18(11);1273-95
[PubMed:22938038] [WorldCat.org] [DOI] (I p)

Hervé Joël Defeu Soufo, Christian Reimold, Uwe Linne, Tobias Knust, Johannes Gescher, Peter L Graumann
Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein.
Proc Natl Acad Sci U S A: 2010, 107(7);3163-8
[PubMed:20133608] [WorldCat.org] [DOI] (I p)

Cédric Absalon, Michal Obuchowski, Edwige Madec, Delphine Delattre, I Barry Holland, Simone J Séror
CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis.
Microbiology (Reading): 2009, 155(Pt 3);932-943
[PubMed:19246764] [WorldCat.org] [DOI] (P p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Christine Eymann, Dörte Becher, Jörg Bernhardt, Katrin Gronau, Anja Klutzny, Michael Hecker
Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.
Proteomics: 2007, 7(19);3509-26
[PubMed:17726680] [WorldCat.org] [DOI] (P p)