TsaE

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  • Description: P-loop ATPase, required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA

Gene name ydiB
Synonyms
Essential no
Product tRNA modification enzyme
Function tRNA modification
Gene expression levels in SubtiExpress: ydiB
Interactions involving this protein in SubtInteract: YdiB
MW, pI 17 kDa, 4.597
Gene length, protein length 474 bp, 158 aa
Immediate neighbours thiL, ydiC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YdiB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YdiB expression.png















Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU05910

Phenotypes of a mutant

reduced growth rate PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed
  • Protein family: UPF0079 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: K(M) for ATP: 60 myM, V(max) 10 nmol/min PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

ATPase activity is required for in vivo function of the protein PubMed

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP833, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP841, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References