Difference between revisions of "TsaE"

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* '''Description:''' write here <br/><br/>
+
* '''Description:''' P-loop ATPase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
+
|style="background:#ABCDEF;" align="center"| '''Product''' || P-loop ATPase
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
reduced growth rate  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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=== Extended information on the protein ===
 
=== Extended information on the protein ===
  
* '''Kinetic information:'''
+
* '''Kinetic information:''' K(M) for ATP: 60 myM, V(max) 10 nmol/min  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
  
 
* '''Domains:'''  
 
* '''Domains:'''  
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
+
* '''Interactions:''' dimerizes and oligomerizes  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
  
* '''Localization:'''
+
* '''Localization:''' cytoplasm with the excepton of the nucleoid region  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 +
 +
ATPase activity is required for ''in vivo'' function of the protein  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
  
 
=Expression and regulation=
 
=Expression and regulation=
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# Hunt, A., Rawlins, J. P., Thomaides, H. B., and Errington, J. (2006) Functional analysis of 11 putative essential genes in Bacillus subtilis. Microbiology 152, 2895-2907. [http://www.ncbi.nlm.nih.gov/sites/entrez/17005971 PubMed]
 
# Hunt, A., Rawlins, J. P., Thomaides, H. B., and Errington, J. (2006) Functional analysis of 11 putative essential genes in Bacillus subtilis. Microbiology 152, 2895-2907. [http://www.ncbi.nlm.nih.gov/sites/entrez/17005971 PubMed]
 +
# Karst JC, Foucher AE, Campbell TL, Di Guilmi AM, Stroebel D, Mangat CS, Brown ED, Jault JM (2009) The ATPase activity of an 'essential' ''Bacillus subtilis'' enzyme, YdiB, is required for its cellular function and is modulated by oligomerization. ''Microbiology'' '''155:''' 944-965. [http://www.ncbi.nlm.nih.gov/sites/entrez/19246765 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 09:39, 3 March 2009

  • Description: P-loop ATPase

Gene name ydiB
Synonyms
Essential no
Product P-loop ATPase
Function unknown
MW, pI 17 kDa, 4.597
Gene length, protein length 474 bp, 158 aa
Immediate neighbours thiL, ydiC
Gene sequence (+200bp) Protein sequence
Genetic context
YdiB context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

reduced growth rate PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: K(M) for ATP: 60 myM, V(max) 10 nmol/min PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: dimerizes and oligomerizes PubMed
  • Localization: cytoplasm with the excepton of the nucleoid region PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

ATPase activity is required for in vivo function of the protein PubMed

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Hunt, A., Rawlins, J. P., Thomaides, H. B., and Errington, J. (2006) Functional analysis of 11 putative essential genes in Bacillus subtilis. Microbiology 152, 2895-2907. PubMed
  2. Karst JC, Foucher AE, Campbell TL, Di Guilmi AM, Stroebel D, Mangat CS, Brown ED, Jault JM (2009) The ATPase activity of an 'essential' Bacillus subtilis enzyme, YdiB, is required for its cellular function and is modulated by oligomerization. Microbiology 155: 944-965. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed