tsaE

tsaE
168

protein kinase, required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA

locus
BSU_05910
Molecular weight
17.76 kDa
pI
4.6
Protein length
Gene length
function
control of tRNA modification
product
protein kinase involved in tRNA modification
essential
no
synonyms
tsaE, ydiB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0802 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
641,654 → 642,130
Phenotypes of a mutant
reduced growth rate  [Pubmed|19246765]
increased sensitivity to paraquat [pubmed|28890133]
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The protein
Catalyzed reaction/ biological activity
biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]) [Pubmed|23072323,21183954]
autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 [pubmed|28890133]
phosphorylation of [protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD] [pubmed|28890133]
Protein family
TsaE family (single member, according to UniProt)
[wiki|Cofactors]
Mg2+ [pubmed|28890133]
Structure
[PDB|5MVR] [Pubmed|28890133]
[AF|O05515]
Modification
autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 [pubmed|28890133]
Kinetic information
K(M) for ATP: 60 myM, V(max) 10 nmol/min  [Pubmed|19246765]
[wiki|Localization]
cytoplasm with the exception of the nucleoid region  [Pubmed|19246765]
Additional information
ATPase activity is required for ''in vivo'' function of the protein  [Pubmed|19246765]
Expression and Regulation
Operons
genes
[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
description
[Pubmed|22383849]
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[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]→[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]

2024-03-10 09:00:43

ghost

87

45839886739c513a86015eb2db5091e043ecd558

F160E3DB3BAB4687DBF8F609242CE813EE9E1BF6

Biological materials
Mutant
BKE05910 (Δ[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGCTTCACACGAGTCACCC,  downstream forward: _UP4_ATGCTTTGTGAGGAGTTAAG
BKK05910 (Δ[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGCTTCACACGAGTCACCC,  downstream forward: _UP4_ATGCTTTGTGAGGAGTTAAG
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP833, available in [wiki|Stülke] lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP841, available in [wiki|Stülke] lab
References
17005971,19246765,23072323,22383849,12112691,28890133,29741707,33044618,34500011

E15BB518649B2B9CD44CEBBB4FA481E55BCE823C

Page visits: 4276

Time of last update: 2024-03-28 07:28:48

Author of last update: Jstuelk