Difference between revisions of "TsaD"

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(Publications on the corresponding E. coli protein, YgjD)
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=References=
 
=References=
 
==Publications on the corresponding'' E. coli'' protein, YgjD==
 
==Publications on the corresponding'' E. coli'' protein, YgjD==
<pubmed> 21183954 19376873 20824107 21619589 </pubmed>
+
<pubmed> 21183954 19376873 20824107 21619589 21285948 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:06, 1 August 2011

  • Description: there are two annotations for the corresponding E. coli protein: O-sialoglycoprotein endopeptidase, and required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA, universally conserved protein

Gene name gcp
Synonyms ydiE
Essential yes PubMed
Product O-sialoglycoprotein endopeptidase (putative)
Function tRNA modification
Interactions involving this protein in SubtInteract: Gcp
MW, pI 36 kDa, 5.016
Gene length, protein length 1038 bp, 346 aa
Immediate neighbours ydiD, ydiF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Gcp context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

translation, essential genes, universally conserved proteins, poorly characterized/ putative enzymes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU05940

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs PubMed
    • conserved glycopeptidase that may be directly involved in the metabolism of glycated proteins PubMed
  • Protein family: peptidase M22 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

universally conserved protein

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Publications on the corresponding E. coli protein, YgjD

Tobias Bergmiller, Rafael Peña-Miller, Alexander Boehm, Martin Ackermann
Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD.
BMC Microbiol: 2011, 11;118
[PubMed:21619589] [WorldCat.org] [DOI] (I e)

Basma El Yacoubi, Isabelle Hatin, Christopher Deutsch, Tamer Kahveci, Jean-Pierre Rousset, Dirk Iwata-Reuyl, Alexey G Murzin, Valérie de Crécy-Lagard
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
EMBO J: 2011, 30(5);882-93
[PubMed:21285948] [WorldCat.org] [DOI] (I p)

Madhusudhan Srinivasan, Preeti Mehta, Yao Yu, Evelyn Prugar, Eugene V Koonin, A Wali Karzai, Rolf Sternglanz
The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A.
EMBO J: 2011, 30(5);873-81
[PubMed:21183954] [WorldCat.org] [DOI] (I p)

Chen Katz, Ifat Cohen-Or, Uri Gophna, Eliora Z Ron
The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins.
mBio: 2010, 1(3);
[PubMed:20824107] [WorldCat.org] [DOI] (I e)

Jennifer I Handford, Bérengère Ize, Grant Buchanan, Gareth P Butland, Jack Greenblatt, Andrew Emili, Tracy Palmer
Conserved network of proteins essential for bacterial viability.
J Bacteriol: 2009, 191(15);4732-49
[PubMed:19376873] [WorldCat.org] [DOI] (I p)