Difference between revisions of "TsaC"

From SubtiWiki
Jump to: navigation, search
Line 48: Line 48:
  
  
 +
 +
= Categories containing this gene/protein =
 +
{{SubtiWiki category|[[translation]]}}
 
=The protein=
 
=The protein=
  

Revision as of 00:02, 1 December 2010

  • Description: biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A)

Gene name ywlC
Synonyms ipc-29d
Essential no
Product unknown
Function tRNA modification
MW, pI 36 kDa, 5.82
Gene length, protein length 1038 bp, 346 aa
Immediate neighbours ywlD, ywlB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwlC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU36950

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

Categories containing this gene/protein

translation

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding E. coli protein YrdC) PubMed
  • Protein family: SUA5 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1HRU (the homolog from E. coli) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Basma El Yacoubi, Benjamin Lyons, Yulien Cruz, Robert Reddy, Brian Nordin, Fabio Agnelli, James R Williamson, Paul Schimmel, Manal A Swairjo, Valérie de Crécy-Lagard
The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA.
Nucleic Acids Res: 2009, 37(9);2894-909
[PubMed:19287007] [WorldCat.org] [DOI] (I p)

Shu Ishikawa, Yoshitoshi Ogura, Mika Yoshimura, Hajime Okumura, Eunha Cho, Yoshikazu Kawai, Ken Kurokawa, Taku Oshima, Naotake Ogasawara
Distribution of stable DnaA-binding sites on the Bacillus subtilis genome detected using a modified ChIP-chip method.
DNA Res: 2007, 14(4);155-68
[PubMed:17932079] [WorldCat.org] [DOI] (P p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)