Difference between revisions of "TrpP"

From SubtiWiki
Jump to: navigation, search
(References)
(Expression and regulation)
Line 92: Line 92:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[trpP]]'' {{PubMed|10735881}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|10735881}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** induced in the absence of tryptophan ([[MtrB]]) {{PubMed|10735881}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[MtrB]]: translation inhibition, the [[MtrB]] binding site overlaps the Shine-DAlgarno sequence and translation initation region, [[MtrB]] binds in the presence of tryptophan {{PubMed|10735881}}
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 19:36, 16 September 2009

  • Description: L-tryptophan transporter (proton symport)

Gene name trpP
Synonyms yhaG
Essential no
Product L-tryptophan transporter (proton symport)
Function tryptophan uptake
Metabolic function and regulation of this protein in SubtiPathways:
Phe, Tyr, Trp
MW, pI 18 kDa, 11.155
Gene length, protein length 516 bp, 172 aa
Immediate neighbours yhaH, serC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YhaG context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU10010

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: trpP transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced in the absence of tryptophan (MtrB) PubMed
  • Regulatory mechanism:
    • MtrB: translation inhibition, the MtrB binding site overlaps the Shine-DAlgarno sequence and translation initation region, MtrB binds in the presence of tryptophan PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Helen Yakhnin, Hong Zhang, Alexander V Yakhnin, Paul Babitzke
The trp RNA-binding attenuation protein of Bacillus subtilis regulates translation of the tryptophan transport gene trpP (yhaG) by blocking ribosome binding.
J Bacteriol: 2004, 186(2);278-86
[PubMed:14702295] [WorldCat.org] [DOI] (P p)

J P Sarsero, E Merino, C Yanofsky
A Bacillus subtilis gene of previously unknown function, yhaG, is translationally regulated by tryptophan-activated TRAP and appears to be involved in tryptophan transport.
J Bacteriol: 2000, 182(8);2329-31
[PubMed:10735881] [WorldCat.org] [DOI] (P p)