trpB

168

tryptophan synthase (beta subunit)

locus: BSU_22640

Molecular weight: 43.42 kDa

pI: 5.53

Protein length: 400 aa

Gene length: 1200 bp

function: biosynthesis of tryptophan

product: tryptophan synthase (beta subunit)

essential: no

ec: 4.2.1.20

synonyms: trpB

List of homologs in different organisms, belongs to COG0133 (Galperin et al., 2021)

Gene

Coordinates: 2,372,304 → 2,373,506

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The protein

Catalyzed reaction/ biological activity

• (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine --> D-glyceraldehyde 3-phosphate + H₂O + L-tryptophan (according to UniProt)

Protein family

• TrpB family (single member, according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|4NEG] (from Bacillus anthracis, 60% identity)
• [AF|P07600]

Biological materials

Mutant

• BKE22640 (Δ[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCCAAAATCACCGTATCTGC,  downstream forward: _UP4_GTATTGGAAGAAGAGGTGAA
• BKK22640 (Δ[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCCAAAATCACCGTATCTGC,  downstream forward: _UP4_GTATTGGAAGAAGAGGTGAA

References

Reviews

• 18486479,11893063,11163353,7900177,19387555,12859215,11395405,12966138

Original Publications

• 14976255,3924737,6436812,2422155,8419914,1551827,17507374,21815947,31871208