TrmU

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  • Description: tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase

Gene name trmU
Synonyms yrrA
Essential yes PubMed
Product tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
Function tRNA modification
Gene expression levels in SubtiExpress: trmU
MW, pI 42 kDa, 4.729
Gene length, protein length 1122 bp, 374 aa
Immediate neighbours yrrB, yrvO
Sequences Protein DNA DNA_with_flanks
Genetic context
TrmU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TrmU expression.png















Categories containing this gene/protein

translation, essential genes, membrane proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU27500

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with YrvO) PubMed
  • Protein family: mnmA/TRMU family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 164 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 304 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Katherine A Black, Patricia C Dos Santos
Abbreviated Pathway for Biosynthesis of 2-Thiouridine in Bacillus subtilis.
J Bacteriol: 2015, 197(11);1952-62
[PubMed:25825430] [WorldCat.org] [DOI] (I p)

Soon-Yong Choi, Dindo Reyes, Montira Leelakriangsak, Peter Zuber
The global regulator Spx functions in the control of organosulfur metabolism in Bacillus subtilis.
J Bacteriol: 2006, 188(16);5741-51
[PubMed:16885442] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)