TopA

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  • Description: DNA topoisomerase I

Gene name topA
Synonyms topI
Essential no
Product DNA topoisomerase I
Function replication
MW, pI 78 kDa, 9.023
Gene length, protein length 2073 bp, 691 aa
Immediate neighbours smf, gid
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
TopA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU16120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
  • Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
  • Paralogous protein(s): TopB, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)