TopA

From SubtiWiki
Revision as of 19:33, 29 April 2013 by Jstuelk (talk | contribs)
Jump to: navigation, search
  • Description: DNA topoisomerase I

Gene name topA
Synonyms topI
Essential no
Product DNA topoisomerase I
Function DNA replication
Gene expression levels in SubtiExpress: topA
MW, pI 78 kDa, 9.023
Gene length, protein length 2073 bp, 691 aa
Immediate neighbours dprA, trmFO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
TopA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TopA expression.png















Categories containing this gene/protein

DNA condensation/ segregation

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU16120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
  • Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
  • Paralogous protein(s): TopB, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: topA (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)