TopA

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  • Description: DNA topoisomerase I

Gene name topA
Synonyms topI
Essential no
Product DNA topoisomerase I
Function DNA replication
Gene expression levels in SubtiExpress: topA
MW, pI 78 kDa, 9.023
Gene length, protein length 2073 bp, 691 aa
Immediate neighbours dprA, trmFO
Sequences Protein DNA DNA_with_flanks
Genetic context
TopA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TopA expression.png















Categories containing this gene/protein

DNA condensation/ segregation

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU16120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
  • Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
  • Paralogous protein(s): TopB, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: topA (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)