Difference between revisions of "TopA"
| Line 8: | Line 8: | ||
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''topI '' | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''topI '' | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || | + | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|25092907}} |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA topoisomerase I | |style="background:#ABCDEF;" align="center"| '''Product''' || DNA topoisomerase I | ||
| Line 40: | Line 40: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[DNA condensation/ segregation]]}} | + | {{SubtiWiki category|[[DNA condensation/ segregation]]}}, |
| + | {{SubtiWiki category|[[essential genes]]}} | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 52: | Line 53: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * essential {{PubMed|25092907}} | ||
=== Database entries === | === Database entries === | ||
| Line 70: | Line 72: | ||
* '''Protein family:''' prokaryotic type I/III topoisomerase family (according to Swiss-Prot) | * '''Protein family:''' prokaryotic type I/III topoisomerase family (according to Swiss-Prot) | ||
| − | * '''Paralogous protein(s):''' [[TopB] | + | * '''Paralogous protein(s):''' [[TopB]] |
=== Extended information on the protein === | === Extended information on the protein === | ||
| Line 138: | Line 140: | ||
=References= | =References= | ||
| − | <pubmed>17114254,21710567 </pubmed> | + | <pubmed>17114254,21710567 25092907</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:26, 6 August 2014
- Description: DNA topoisomerase I
| Gene name | topA |
| Synonyms | topI |
| Essential | yes PubMed |
| Product | DNA topoisomerase I |
| Function | DNA replication |
| Gene expression levels in SubtiExpress: topA | |
| MW, pI | 78 kDa, 9.023 |
| Gene length, protein length | 2073 bp, 691 aa |
| Immediate neighbours | dprA, trmFO |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA condensation/ segregation, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16120
Phenotypes of a mutant
- essential PubMed
Database entries
- BsubCyc: BSU16120
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
- Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
- Paralogous protein(s): TopB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- TopA interacts with the RNA polymerase PubMed
Database entries
- BsubCyc: BSU16120
- Structure:
- UniProt: P39814
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: topA (according to DBTBS)
- Regulation:
- Regulatory mechanism:
- Additional information:
- translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 324 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 1123 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Mario Juhas, Daniel R Reuß, Bingyao Zhu, Fabian M Commichau
Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering.
Microbiology (Reading): 2014, 160(Pt 11);2341-2351
[PubMed:25092907]
[WorldCat.org]
[DOI]
(I p)
Olivier Delumeau, François Lecointe, Jan Muntel, Alain Guillot, Eric Guédon, Véronique Monnet, Michael Hecker, Dörte Becher, Patrice Polard, Philippe Noirot
The dynamic protein partnership of RNA polymerase in Bacillus subtilis.
Proteomics: 2011, 11(15);2992-3001
[PubMed:21710567]
[WorldCat.org]
[DOI]
(I p)
Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254]
[WorldCat.org]
[DOI]
(P p)
