Difference between revisions of "TopA"
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA topoisomerase I | |style="background:#ABCDEF;" align="center"| '''Product''' || DNA topoisomerase I | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || replication | + | |style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA replication]] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU16120 topA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU16120 topA] | ||
| Line 37: | Line 37: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<br/><br/> | <br/><br/> | ||
| Line 47: | Line 43: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| − | + | [[Efp-dependent proteins]] | |
=The gene= | =The gene= | ||
| Line 110: | Line 106: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=topA_1683661_1685736_1 topA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=topA_1683661_1685736_1 topA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 117: | Line 113: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** [[translation]] is likely to require [[Efp]] due to the presence of several consecutive proline residues {{PubMed|23239624,23239623}} | ||
=Biological materials = | =Biological materials = | ||
Revision as of 19:33, 29 April 2013
- Description: DNA topoisomerase I
| Gene name | topA |
| Synonyms | topI |
| Essential | no |
| Product | DNA topoisomerase I |
| Function | DNA replication |
| Gene expression levels in SubtiExpress: topA | |
| MW, pI | 78 kDa, 9.023 |
| Gene length, protein length | 2073 bp, 691 aa |
| Immediate neighbours | dprA, trmFO |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16120
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
- Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- TopA interacts with the RNA polymerase PubMed
Database entries
- Structure:
- UniProt: P39814
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: topA (according to DBTBS)
- Regulation:
- Regulatory mechanism:
- Additional information:
- translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254]
[WorldCat.org]
[DOI]
(P p)
