tmk

168

thymidylate kinase

locus: BSU_00280

Molecular weight: 23.73 kDa

pI: 4.87

Protein length: 212 aa

Gene length: 639 bp

function: TTP biosynthesis

product: thymidylate kinase

essential: yes

ec: 2.7.4.9

synonyms: tmk, yaaP

List of homologs in different organisms, belongs to COG0125 (Galperin et al., 2021)

Gene

Coordinates: 39,159  39,797

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• ATP +  dTMP --> ADP +  dTDP (according to UniProt)

Protein family

• Thymidylate kinase family (single member, according to UniProt)

Structure

• [PDB|2CCK] (from ''Staphylococcus aureus'', 53% identity, 74% similarity) [Pubmed|16522804]
• [AF|P37537]

[wiki|Localization]

• cytoplasm (according to UniProt)

Biological materials

Expression vectors

• pGP1689: expression of Strep-''tmk'' in ''E. coli'', in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
• pGP2671: expression of ''tmk''-Strep, in [wiki|pGP380] ,in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

FLAG-tag construct

• GP2401 ''tmk-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab

References

• 16522804,18523102,23043329,18523102,27376462,23394555