Difference between revisions of "Tmk"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
Line 133: Line 133:
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:'''  
+
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 11:23, 1 April 2015

  • Description: thymidylate kinase

Gene name tmk
Synonyms yaaP
Essential yes PubMed
Product thymidylate kinase
Function TTP biosynthesis
Gene expression levels in SubtiExpress: tmk
Metabolic function and regulation of this protein in SubtiPathways:
tmk
MW, pI 23 kDa, 4.866
Gene length, protein length 636 bp, 212 aa
Immediate neighbours yaaO, darA
Sequences Protein DNA DNA_with_flanks
Genetic context
Tmk context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Tmk expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00280

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + dTMP = ADP + dTDP (according to Swiss-Prot)
  • Protein family: thymidylate kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2CCK (from Staphylococcus aureus, 53% identity, 74% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Masayo Kotaka, Balvinder Dhaliwal, Jingshan Ren, Charles E Nichols, Richard Angell, Michael Lockyer, Alastair R Hawkins, David K Stammers
Structures of S. aureus thymidylate kinase reveal an atypical active site configuration and an intermediate conformational state upon substrate binding.
Protein Sci: 2006, 15(4);774-84
[PubMed:16522804] [WorldCat.org] [DOI] (P p)