TlpA

• Description: membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein
  
  

Gene name	tlpA
Synonyms
Essential	no
Product	methyl-accepting chemotaxis protein
Function	control of chemotaxis
Gene expression levels in SubtiExpress: tlpA
MW, pI	72 kDa, 4.906
Gene length, protein length	1986 bp, 662 aa
Immediate neighbours	mcpA, mcpB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigD regulon

The gene

Basic information

• Locus tag: BSU31250

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane PubMed, Polar (Septum) PubMed

Database entries

• Structure:

• UniProt: P39216

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tlpA-mcpA PubMed

• Expression browser: tlpA PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:
  • in minimal medium, TlpA is present with 2,100 +/- 890 molecules per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

D W Hanlon, G W Ordal
Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis.
J Biol Chem: 1994, 269(19);14038-46
[PubMed:8188684] [WorldCat.org] (P p)