thrB

168

homoserine kinase

locus: BSU_32240

Molecular weight: 33.17 kDa

pI: 4.74

Protein length: 309 aa

Gene length: 930 bp

function: biosynthesis of threonine

product: homoserine kinase

essential: no

ec: 2.7.1.39

synonyms: thrB, thrA

List of homologs in different organisms, belongs to COG0083 (Galperin et al., 2021)

Gene

Coordinates: 3,312,844  3,313,773

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The protein

Catalyzed reaction/ biological activity

• ATP + L-homoserine --> ADP + H⁺ + O-phospho-L-homoserine (according to UniProt)

Protein family

• GHMP kinase family (together with [protein|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|galK] and [protein|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]) (according to UniProt)

Structure

• [PDB|3HUL] (from ''Listeria monocytogenes'', 41% identity, 61% similarity)
• [AF|P04948]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE32240 ([gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGAGAACAGCATGTCGGCTT,  downstream forward: _UP4_TAGATCATGCCAAGCGTTCC
• BKK32240 ([gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGAGAACAGCATGTCGGCTT,  downstream forward: _UP4_TAGATCATGCCAAGCGTTCC
• BP1275 ([gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]::phleo trp+) available in [wiki|Jörg Stülke]'s & [wiki|Fabian Commichau]'s lab

References

• 12107147,3098560,24163341,27260660