Difference between revisions of "ThrB"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
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{{SubtiWiki regulon|[[CodY regulon]]}}
  
 
=The gene=
 
=The gene=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thrB_3312844_3313773_-1 thrB] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thrB_3312844_3313773_-1 thrB] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' repressed by casamino acids {{PubMed|12107147}}   
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* '''Regulation:'''  
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** repressed by casamino acids {{PubMed|12107147}}   
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** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|24163341}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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** [[CodY]]: transcription repression {{PubMed|24163341}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>12107147 3098560, </pubmed>
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<pubmed>12107147 3098560,24163341 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:06, 22 December 2013

  • Description: homoserine kinase

Gene name thrB
Synonyms thrA
Essential no
Product homoserine kinase
Function biosynthesis of threonine
Gene expression levels in SubtiExpress: thrB
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr
MW, pI 33 kDa, 4.735
Gene length, protein length 927 bp, 309 aa
Immediate neighbours yuxL, thrC
Sequences Protein DNA DNA_with_flanks
Genetic context
ThrB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThrB expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

  • Locus tag: BSU32240

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-homoserine = ADP + O-phospho-L-homoserine (according to Swiss-Prot)
  • Protein family: Homoserine kinase subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 3HUL (from Listeria monocytogenes, 41% identity, 61% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Allison Kriel, Shaun R Brinsmade, Jessica L Tse, Ashley K Tehranchi, Alycia N Bittner, Abraham L Sonenshein, Jue D Wang
GTP dysregulation in Bacillus subtilis cells lacking (p)ppGpp results in phenotypic amino acid auxotrophy and failure to adapt to nutrient downshift and regulate biosynthesis genes.
J Bacteriol: 2014, 196(1);189-201
[PubMed:24163341] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

C Parsot
Evolution of biosynthetic pathways: a common ancestor for threonine synthase, threonine dehydratase and D-serine dehydratase.
EMBO J: 1986, 5(11);3013-9
[PubMed:3098560] [WorldCat.org] [DOI] (P p)