Difference between revisions of "ThiW"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 643 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Latest revision as of 10:10, 17 April 2014

Gene name thiW
Synonyms ykoD
Essential no
Product thiamine ABC transporter (ATP-binding protein)
Function thiamine uptake
Gene expression levels in SubtiExpress: thiW
Interactions involving this protein in SubtInteract: ThiW
Metabolic function and regulation of this protein in SubtiPathways:
thiW
MW, pI 54 kDa, 5.708
Gene length, protein length 1470 bp, 490 aa
Immediate neighbours thiX, thiV
Sequences Protein DNA DNA_with_flanks
Genetic context
YkoD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiW expression.png




























Categories containing this gene/protein

ABC transporters, biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

  • Locus tag: BSU13220

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Additional information:
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 643 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.
J Biol Chem: 2002, 277(50);48949-59
[PubMed:12376536] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)