Difference between revisions of "ThiU"

From SubtiWiki
Jump to: navigation, search
Line 90: Line 90:
 
* '''Operon:'''  
 
* '''Operon:'''  
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15699190}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 15:33, 31 July 2009

  • Description: thiamine ABC transporter (binding protein)

Gene name thiU
Synonyms ykoF
Essential no
Product thiamine ABC transporter (binding protein)
Function thiamine uptake
MW, pI 21 kDa, 6.046
Gene length, protein length 600 bp, 200 aa
Immediate neighbours thiV, ykoG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkoF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU13240

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1SBR (complex with thiamin), 1S99
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Yancho Devedjiev, Yogesh Surendranath, Urszula Derewenda, Alexandra Gabrys, David R Cooper, Rong-guang Zhang, Lour Lezondra, Andrzej Joachimiak, Zygmunt S Derewenda
The structure and ligand binding properties of the B. subtilis YkoF gene product, a member of a novel family of thiamin/HMP-binding proteins.
J Mol Biol: 2004, 343(2);395-406
[PubMed:15451668] [WorldCat.org] [DOI] (P p)