Difference between revisions of "ThiS"

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(References)
(Expression and regulation)
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* '''Regulation:'''  
 
* '''Regulation:'''  
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** repressed by thiamine ([[Thi-box]]) {{PubMed| 16356850}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  

Revision as of 14:14, 24 November 2009

  • Description: hydroxyethylthiazole phosphate biosynthesis

Gene name thiS
Synonyms yjbS
Essential no
Product unknown
Function biosynthesis of thiamine
MW, pI 7 kDa, 5.501
Gene length, protein length 198 bp, 66 aa
Immediate neighbours thiO, thiG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThiS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU11680

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ethan C Settembre, Pieter C Dorrestein, Huili Zhai, Abhishek Chatterjee, Fred W McLafferty, Tadhg P Begley, Steven E Ealick
Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex.
Biochemistry: 2004, 43(37);11647-57
[PubMed:15362849] [WorldCat.org] [DOI] (P p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)