Difference between revisions of "ThiO"

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(Expression and regulation)
(Expression and regulation)
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* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed by thiamine ([[Thi-box]]) {{PubMed| 16356850}}
+
** repressed by thiamine ([[Thi-box]]) {{PubMed|16356850}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[Thi-box]]: [[RNA switch]] {{PubMed| 16356850}}
+
** [[Thi-box]]: [[RNA switch]] {{PubMed|16356850}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 14:36, 24 November 2009

  • Description: FAD-dependent glycine oxidase

Gene name thiO
Synonyms goxB, yjbR
Essential no
Product glycine oxidase
Function biosynthesis of thiamine
MW, pI 40 kDa, 5.898
Gene length, protein length 1107 bp, 369 aa
Immediate neighbours tenI, thiS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GoxB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU11670

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Glycine + H2O + O2 = glyoxylate + NH3 + H2O2 (according to Swiss-Prot)
  • Protein family: DAMOX/DASOX family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: the FAD-containing holoenzyme is a homotetramer PubMed
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1NG3 (complex with acetyl-glycine), 1RYI
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mattia Pedotti, Elena Rosini, Gianluca Molla, Tommaso Moschetti, Carmelinda Savino, Beatrice Vallone, Loredano Pollegioni
Glyphosate resistance by engineering the flavoenzyme glycine oxidase.
J Biol Chem: 2009, 284(52);36415-36423
[PubMed:19864430] [WorldCat.org] [DOI] (I p)

Laura Caldinelli, Mattia Pedotti, Laura Motteran, Gianluca Molla, Loredano Pollegioni
FAD binding in glycine oxidase from Bacillus subtilis.
Biochimie: 2009, 91(11-12);1499-508
[PubMed:19751796] [WorldCat.org] [DOI] (I p)

Mattia Pedotti, Sandro Ghisla, Laura Motteran, Gianluca Molla, Loredano Pollegioni
Catalytic and redox properties of glycine oxidase from Bacillus subtilis.
Biochimie: 2009, 91(5);604-12
[PubMed:19254749] [WorldCat.org] [DOI] (I p)

Mario Mörtl, Kay Diederichs, Wolfram Welte, Gianluca Molla, Laura Motteran, Gabriella Andriolo, Mirella S Pilone, Loredano Pollegioni
Structure-function correlation in glycine oxidase from Bacillus subtilis.
J Biol Chem: 2004, 279(28);29718-27
[PubMed:15105420] [WorldCat.org] [DOI] (P p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)

Gianluca Molla, Laura Motteran, Viviana Job, Mirella S Pilone, Loredano Pollegioni
Kinetic mechanisms of glycine oxidase from Bacillus subtilis.
Eur J Biochem: 2003, 270(7);1474-82
[PubMed:12654003] [WorldCat.org] [DOI] (P p)

Ethan C Settembre, Pieter C Dorrestein, Joo-Heon Park, Amy M Augustine, Tadhg P Begley, Steven E Ealick
Structural and mechanistic studies on ThiO, a glycine oxidase essential for thiamin biosynthesis in Bacillus subtilis.
Biochemistry: 2003, 42(10);2971-81
[PubMed:12627963] [WorldCat.org] [DOI] (P p)

Viviana Job, Giorgia Letizia Marcone, Mirella S Pilone, Loredano Pollegioni
Glycine oxidase from Bacillus subtilis. Characterization of a new flavoprotein.
J Biol Chem: 2002, 277(9);6985-93
[PubMed:11744710] [WorldCat.org] [DOI] (P p)

Y Nishiya, T Imanaka
Purification and characterization of a novel glycine oxidase from Bacillus subtilis.
FEBS Lett: 1998, 438(3);263-6
[PubMed:9827558] [WorldCat.org] [DOI] (P p)